Supplementary Table 1
Temperature at loss of equilibrium (LOE) of Kryptolebias marmoratus acclimated to
25°C or 30°C for one week. Temperature was increased by 0.3°C/min. There was a significant
difference between treatment groups (Student's t-test, p = 0.0003).
(a) Percentage of immersed (submerged) Kryptolebias marmoratus (control, reared and acclimated to 25°C) when exposed to rising water temperatures (approx. 1°C min−1). The line of best fit follows the sigmoidal curve (b) Emersion temperatures and (c) loss of equilibrium (LOE) temperatures of K. marmoratus reared or acclimated to 25 or 30°C. Significant differences are indicated by an asterisk (means ± s.e.). Sample sizes are given in parentheses.
(a) Representative thermal images showing the decrease in temperature of Kryptolebias marmoratus (arrows) after 30 and 60 s of emersion. W, water; fp, filter paper. Scale bar, 5 cm. (b) Mean (±s.e.) body temperature of emersed fish in high and low humidity treatments (each n = 7). Starting water temperature was 25°C and air temperature was constant at 30°C. Different letters indicate significant differences within a treatment over time (p < 0.001). Asterisks denote significant differences between treatments (p < 0.01).